| Autor: |
Suva Roy, Depeng Wang, Andra M. Rudzite, Benjamin Perry, Miranda L. Scalabrino, Mishek Thapa, Yiyang Gong, Alexander Sher, Greg D. Field |
| Rok vydání: |
2022 |
| DOI: |
10.1101/2022.09.26.508527 |
| Popis: |
Visual processing in the retina depends on the collective activity of large ensembles of neurons organized in different layers. Current techniques for measuring activity of layer-specific neural ensembles rely on expensive pulsed infrared lasers to drive 2-photon activation of calcium-dependent fluorescent reporters. Here, we present a 1-photon light-sheet imaging system that can measure the activity in hundreds of ex vivo retinal neurons over a large field of view while simultaneously presenting visual stimuli. This allowed for a reliable functional classification of different retinal ganglion cell types. We also demonstrate that the system has sufficient resolution to image calcium entry at individual synaptic release sites across the axon terminals of dozens of simultaneously imaged bipolar cells. The simple design, a large field of view, and fast image acquisition, make this a powerful system for high-throughput and high-resolution measurements of retinal processing at a fraction of the cost of alternative approaches. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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