Production of transgenic maize plants and progeny by bombardment of hi-II immature embryos

Autor:	D. D. Songstad, Maud A. W. Hinchee, B. Hairston, William L. Petersen, Charles L. Armstrong
Rok vydání:	1996
Předmět:	Genetically modified maize Cell division Transgene fungi food and beverages Embryo Plant Science Genetically modified crops Biology Scutellum Horticulture Transformation (genetics) Callus Botany Biotechnology
Zdroj:	In Vitro Cellular & Developmental Biology - Plant. 32:179-183
ISSN:	1475-2689 1054-5476
Popis:	Production of transgenic maize (Zea mays L.) callus, plants, and progeny from microprojectile bombardment of 2–5-d cultured Hi-II immature embryos is described. Histological evidence indicates that these tissues are amenable to transformation due to surface layer cell division of the scutellum. Two out of every 100 bombarded embryos produced transgenic callus and R0 transgenic plants were both male and female fertile. Expected segregation of transgenes was observed in progeny. The primary advantage of bombarding these tissues is increased male and female fertility of transgenic plants compared with those produced using long-term callus or suspension cultures.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::13792ce2c5d0ea6c27bee5983cce24ed https://doi.org/10.1007/bf02822763 Zobrazit plný text záznamu Full text from SpringerLink