Molecular characterization and expression ofKiss2/Kiss2rduring embryonic and larval development in (Megalobrama amblycephalaYih, 1955)
| Autor: | G.-L. Ruan, Weimin Wang, Jiufu Wen, Zexia Gao, Honghao Zhao |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
biology Embryogenesis Embryo Aquatic Science biology.organism_classification Molecular biology 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Rapid amplification of cDNA ends Complementary DNA Gene expression medicine Blastocyst Gene Megalobrama |
| Zdroj: | Journal of Applied Ichthyology. 32:288-295 |
| ISSN: | 0175-8659 |
| DOI: | 10.1111/jai.13008 |
| Popis: | Summary In order to test whether Kiss/Kissr systems have potential roles in regulating the embryonic and larval development in teleosts, in this study the Kiss2/Kiss2r full-length cDNA was cloned from blunt snout bream (Megalobrama amblycephala Yih, 1955) by rapid amplification of cDNA ends and their expression patterns were detected in different tissues of adult and developmental stages for the embryonic and larval periods via quantitative real-time PCR. Both Kiss and Kissr genes full-length cDNA sequences of M. amblycephala were obtained and phylogenetic analysis results indicated that these genes belong to the Kiss2/Kiss2r clade. Bioinformatics analyses revealed that there was a conserved decapeptide in M. amblycephala Kiss2 gene putative amino acids, but only two transmembrane domains were predicated in Kiss2r. Tissue distribution analyses showed that both genes were widely expressed in the tissues tested, with high levels in the muscle, gonad and pituitary. At different developmental stages, the mRNA expression of Kiss2/Kiss2r was highest in the blastocyst/15 hpf stage and lowest in the 30 hpf /blastocyst stages for the embryonic period, highest in 7 dph/15 dph and lowest in 30 dph/30 dph for larval period, respectively. These results suggest that the Kiss2/Kiss2r system has varied potential for influencing embryonic and larval development in fish species. |
| Databáze: | OpenAIRE |
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