vicK Gene as Potential Identification Marker for Staphylococcus aureus
| Autor: | Nurmusfirah Morad, Suzana Misbah |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
010401 analytical chemistry
Human pathogen 04 agricultural and veterinary sciences Biology medicine.disease_cause 040401 food science 01 natural sciences 0104 chemical sciences law.invention Microbiology 0404 agricultural biotechnology law Staphylococcus aureus Antibiotic therapy medicine Identification (biology) Staphylococcus Gene Polymerase chain reaction Molecular identification |
| Zdroj: | Journal of Biosciences and Medicines. :98-110 |
| ISSN: | 2327-509X 2327-5081 |
| Popis: | Staphylococcus aureus is an important human pathogen frequently detected in hospital community and has emerged as an important health concern in human medicine. Identification of S. aureus from clinical specimens by phenotypic methods may produce variable characteristics leading to ambiguity. Hence, a rapid and reliable method for identification of S. aureus is required which could expedite appropriate antibiotic therapy. This study aimed to evaluate the specificity of polymerase chain reaction (PCR) targeting a signal transduction gene, vicK, among S. aureus isolates of Hospital Sultanah Nur Zahirah, Kuala Terengganu, Malaysia. A total of 118 bacterial isolates were screened, which consisted of one hundred S. aureus isolates, ten Staphylococcus spp. and eight non-Staphylococci. Results indicated that PCR targeting vicK was able to identify 98% of S. aureus isolates with high sensitivity and specificity, while the remaining isolates of Staphylococcus spp. and non-Staphylococci did not yield any amplification of the gene. vicK thus, is highly specific within interspecies and intraspecies, which is potential to be used as a molecular identification marker for S. aureus. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|