Sphingolipidomic analysis in acne vulgaris patient serum shows decreased C24-ceramide and increased ceramide-1-phosphate levels
| Autor: | Sabriye Kaya, Filiz Ozcan, Mutay Aslan, Taner Karaarslan, Ibrahim Aslan, Ebru Kirac |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
medicine.medical_specialty
Ceramide business.industry Selected reaction monitoring Tandem mass spectrometry medicine.disease Biochemistry Sphingolipid Pathophysiology chemistry.chemical_compound Endocrinology chemistry Physiology (medical) Internal medicine medicine lipids (amino acids peptides and proteins) Sphingomyelin business Acne Ceramide 1-phosphate |
| Zdroj: | Free Radical Biology and Medicine. 120:S73-S74 |
| ISSN: | 0891-5849 |
| Popis: | This study aimed to identify circulating levels of neutral sphingomyelinase activity (N-SMase), ceramide-1-phosphate (C1P), sphingosine-1-phosphate (S1P), C16-C24 sphingomyelin (SM), C16-C24 ceramide (CER) in acne vulgaris (AV) patients and controls. Serum was collected from AV patients and age, gender matched controls. Serum levels of C16-C24 SMs and C16-C24 CERs were determined by multiple reaction monitoring (MRM) method using ultrafast-liquid chromatography coupled with tandem mass spectrometry. Serum activity of N-SMase was assayed by standard kit methods, C1P and S1P levels were determined by enzyme-linked immunosorbent assay (ELISA). A significant increase was observed in serum levels of C16 SM in patients with AV compared to controls. Very-long-chain C24 CER was significantly decreased in AV patients compared to controls. A significant positive correlation was found between serum total cholesterol levels and all measured SMs and CERs in both the control and patient groups. Patients with AV had increased circulating levels of C16 SM, C1P and lower circulating levels of C24 CER compared to healthy controls, which may provide prognostic value for the disease. Future studies are needed to understand the role of altered sphingolipid levels in the pathophysiology of AV. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect