Some biochemical aspects of the anaerobic degradation of dairy wastewater

Autor: Gedaliah Shelef, Michal Perle, Shlomo Kimchie
Rok vydání: 1995
Předmět:
Zdroj: Water Research. 29:1549-1554
ISSN: 0043-1354
DOI: 10.1016/0043-1354(94)00248-6
Popis: Low rate of anaerobic degradation together with inhibition problems caused by components such as milk fat and proteins were found to be reasons for restricted activity of high-rate anaerobic reactors operated with dairy effluent. The present research was aimed to investigate the influence of some relevant compounds, such as casein and milk fat and their degradation compounds, on process efficiency. Methanogenic activity and ATP content of the sludge were used as major follow up criteria for evaluating the influence of the tested compounds on process efficiency. It was found that casein was not degraded by anaerobic cultures which were not pre-acclimated for this compound. On the other hand, cultures acclimated to casein, utilized it very efficiently. Amino acids typical for casein degradation were also used efficiently with no inhibitory effects under the test conditions and with no need for culture pre-acclimatization. Milk fat was found to be a major cause for inhibition of methanogenic activity. It was found to have an immediate influence on reducing gas production rate in reactors to which it was added. Milk fat was also found to have long term effects: sludge pre-exposed to milk fat exhibited reduced methanogenic activity when tested in medium free of milk fat. Milk fat also reduced the ATP concentration in the sludge, indicating reduction of its total physiological activity. Oleic acid, which is a major derivative of milk fat hydrolysis was found to have inhibitory effects on methane production and on ATP concentration. According to the results of the present research, it would be advisable to treat dairy effluents by anaerobic digestion only after reduction of the milk fat concentration below 100 mg/L, and after careful acclimatization of the digester culture to casein.
Databáze: OpenAIRE