RP-HPLC-PDAAnalyses of Tapentadol: Application of Experimental Design
| Autor: | Digvijay Singh, Mrunal U. Patil, Saurabh B. Ganorkar, Kamlendra Singh Bhadoriya, Mukesh C. Sharma, Dinesh M. Dhumal |
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| Rok vydání: | 2016 |
| Předmět: |
Chromatography
Central composite design 010405 organic chemistry Potassium 010401 analytical chemistry Phosphate buffered saline Analytical chemistry chemistry.chemical_element Tapentadol 01 natural sciences 0104 chemical sciences Volumetric flow rate chemistry.chemical_compound chemistry Phase (matter) medicine Methanol Response surface methodology Earth-Surface Processes medicine.drug |
| Zdroj: | Analytical Chemistry Letters. 6:214-223 |
| ISSN: | 2230-7532 2229-7928 |
| DOI: | 10.1080/22297928.2015.1068705 |
| Popis: | A simple, rapid and sensitive RP-HPLC-PDA method was developed and validated as per ICH guidelines for the analysis of tapentadol in bulk as well as in tablet formulation. The RP-HPLC isocratic method was developed using a column LC-GC Qualisil BDS C-18 (250 mm × 4.6 mm × 5 μm) and methanol - potassium dihydrogen phosphate buffer (pH 7.0) mixture (80:20, v/v) as a mobile phase. The flow rate was retained at 1 ml min-1 and the response was detected at 274 nm. Robustness of the developed method was evaluated using two experimental designs (central composite design and response surface methodology). The effect of factors such as mobile phase composition, flow rate and pH of mobile phase were studied. |
| Databáze: | OpenAIRE |
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