Association of DNA damage with vitamin D and hair heavy metals of obese women

Autor: Normina Ahmad Bustami, Farahnaz Amini, Chiat Yin Ng, Soma Mitra, Pui Yee Tan, Eugenie Sin Sing Tan
Rok vydání: 2021
Předmět:
Zdroj: Molecular & Cellular Toxicology. 17:429-438
ISSN: 2092-8467
1738-642X
DOI: 10.1007/s13273-021-00149-2
Popis: Obesity has been linked to DNA damage. The modifiable risk factors may modulate the impact of obesity on DNA damage. This study aimed to assess DNA damage and its association with dietary nutrient, serum 25-hydroxyvitamin D (25(OH)D) and concentration of hair heavy metals of obese and non-obese women. A case–control study was conducted involving 134 women aged between 20 and 50 years. Serum 25(OH)D, fasting glucose, and lipid profile were assessed. Indicators of DNA damage such as percentage of tail DNA, tail moment, tail olive moment, tail intensity and tail length were measured using an alkaline-comet assay. Concentrations of hair heavy metals were quantified using inductively coupled plasma–mass spectrometry (ICP–MS). Participants' daily energy, macro, and micronutrient intake were collected using the Food Frequency Questionnaire. Mean values of serum 25(OH)D was 31.8 ± 0.9 nmol/L. 96.3% of participants were vitamin D deficiency ( 0.05). Obese women with serum 25(OH)D level of ≥ 31 nmol/L had a significantly lower tail moment (p = 0.029) and tail olive moment (p = 0.031); thus, indicating less DNA damage. Additionally, obese women with hair chromium concentration of ≥ 5.88 mg/kg had a significantly higher tail moment (p = 0.047), indicating more DNA damage. DNA damage among obese women correlated with serum 25(OH)D and hair chromium.
Databáze: OpenAIRE
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