Reduced immunoreactivity of urinary albumin in patients with cardiovascular diseases: Analysis of immunochemically nonreactive albumin

Autor:	Kenji Sato, Tokuhiro Kawara, Takeshi Kasama, Toshiaki Baba, Hiroshi Yoshida, Kiyoko Shiba, Aki Nakayama, Jyunichi Nishimaki, Mitsuaki Isobe
Rok vydání:	2008
Předmět:	Chromatography medicine.diagnostic_test biology medicine.drug_class Chemistry Albumin Urine Monoclonal antibody Human serum albumin High-performance liquid chromatography Polyclonal antibodies Immunoassay medicine biology.protein Bovine serum albumin medicine.drug
Zdroj:	Journal of Electrophoresis. 52:57-63
ISSN:	1349-9408 1349-9394
Popis:	We analyzed 55 spot urine samples from patients with cardiovascular diseases. Urinary albumin concentrations were measured with size exclusion high performance liquid chromatography (HPLC), turbidimetric immunoassay (TIA) using anti human serum albumin polyclonal antibody, and enzyme-linked immunosorbent assay (ELISA) using anti human serum albumin monoclonal antibody. Fractionated urine samples from the HPLC were also analyzed with the immunoprecipitation reactions using as same monoclonal antibody as ELISA. As a result, the urinary albumin concentration analyzed by the HPLC was systematically higher than that of immunoassays, however, ‘albumin peak’ from the HPLC contained other urinary proteins. Result of immunoprecipitation reaction showed the presence of monomer albumin that could not react with the monoclonal antibody. These results suggest that not only contamination of other proteins, the albumin fraction from the HPLC included albumin with reduced its reactivity to the specific monoclonal antibody.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::133317cc818edc49758c447cfe97b952 https://doi.org/10.2198/jelectroph.52.57 Zobrazit plný text záznamu