Accessory Growth Factor Requirements of Some Representatives of the Brucella Group

Autor:	Albert Dorfman, Stewart A. Koser, Beverly B. Breslove
Rok vydání:	1941
Předmět:	chemistry.chemical_classification Culture mediums biology Growth factor medicine.medical_treatment Vitamin b complex Brucella biology.organism_classification Yeast Amino acid Microbiology Infectious Diseases chemistry Biochemistry Pantothenic acid medicine Immunology and Allergy Asparagine
Zdroj:	Journal of Infectious Diseases. 69:114-124
ISSN:	1537-6613 0022-1899
DOI:	10.1093/infdis/69.2.114
Popis:	There is little information concerning the accessory growth factor requirements of the Brucella group. In culture mediums composed solely of amino acids, salts, and a non-nitrogenous source of energy, most strains of Brucella either are unable to grow or grow poorly if a large inoculum is used.1,2 The few reports of partial success in cultivating Brucella in synthetic mediums2 have employed one or more of the following technics: a large inoculum, "training," or the use of asparagine, glycerol or lactate, compounds which ordinarily contain growth-promoting substances as impurities. This failure of Brucella to develop in mediums of known chemical composition presents a decided contrast to the prompt growth in infusions of meat, liver and yeast. These sources contain something necessary for growth but the nature of the substances needed and the exact requirements have remained obscure. Preliminary studies have shown that the growth-promoting principles in tissues and yeast may be separated to some extent from other materials and partially purified.3 Kerby4 reported that addition of nicotinic acid and thiamin
Databáze:	OpenAIRE
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