Pollen-mediated flow of bar gene in transgenic herbicide-resistant turf grass Zoysia japonica
Autor: | Jaechun Lee, Pill-Soon Song, Tae-Woong Bae, Hong-Gyu Kang, In-Ja Song, Pyung Ok Lim, Hyo-Yeon Lee, Ok-Chul Chung, Kee Woong Park, Yong-Eok Lee, Hyeon-Jin Sun |
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Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
0301 basic medicine Zoysia japonica Transgene food and beverages Context (language use) Plant Science Biology medicine.disease_cause Weed control 01 natural sciences Gene flow 03 medical and health sciences Horticulture 030104 developmental biology Anthesis Germination Pollen medicine 010606 plant biology & botany Biotechnology |
Zdroj: | Plant Biotechnology Reports. 15:241-250 |
ISSN: | 1863-5474 1863-5466 |
Popis: | Weed control can be most effectively achieved through the use of herbicide-resistance transgene. A preliminary study bar-transgenic Zoysia japonica posed no serious risk on the unintended escape of the transgene from its cultivation site. The present follow-up investigation of the dispersion of pollen and its short-distance escape outside of the bar-transgenic Zoysia japonica habitats were ascertained in terms of environmental factors affecting anthesis and pollen viability. In a 24-h day cycle, zoysiagrass pollen was released predominantly between 08:00 and 10:00, and the pollen was most viable during the same time interval. Optimal temperature and humidity for pollen viability was 15–20 °C and 80–90%, respectively. The pollen germinated in 120 min after anthesis, but under cloudy conditions germination time doubled. No differences in pollen viability/longevity between the transgenic and non-transgenic plants were observed. The pollen-mediated gene flow of transgenic Zoysia japonica to wild-type non-transgenic zoysiagrass species was monitored by measuring the cross-over rate of the bar gene in the context of three different models. At distances within 5 m, the rate of gene flow ranged from 3 to 5.7% according to the models used. The greater the distance from the transgenic plant site, the lower the gene flow rate. The furthest transgene detected was 38 m away and exhibited a 0.25% gene flow rate. The radial model yielded a 3.7% escape rate within a 3 m radius and was wind direction dependent. The distance- and direction-dependent gene flow events were influenced by wind direction and velocity during flowering season. |
Databáze: | OpenAIRE |
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