| Autor: |
Yusuf Christian El-Khaled, Florian Roth, Nils Rädecker, Najeh Kharbatia, Burton H. Jones, Christian R Voolstra, Christian Wild |
| Rok vydání: |
2019 |
| DOI: |
10.17504/protocols.io.9ith4en |
| Popis: |
Knowledge about nitrogen (N) cycling in aquatic ecosystems is limited. While the dinitrogen fixation (N2-fix) process is comparably well studied, the contrasting denitrification (DENI) pathway is highly under-investigated, because of expensive and relatively complex experimental techniques currently available. Thus, we present a combined acetylene assay termed COBRA (= combined blockage/reduction acetylene assay) for the simultaneous determination of N2-fix and DENI in aquatic samples. The technique measures gaseous headspace samples via gas chromatography. Both target gases that accumulate (ethylene for N2-fix, nitrous oxide for DENI quantification) during incubations can be detected and quantified in a single assay and from a single sample. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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