Thermostable exo-inulinase production by semicontinuous cultivation of membrane-immobilized Bacillus sp. 11 cells

Autor:	D. Spasova, K. Uzunova, Anna Vassileva, Alexandra Tonkova, Viara Ivanova
Rok vydání:	2002
Předmět:	chemistry.chemical_classification Thermophile Inulin Membrane structure Bioengineering Bacillus sp Biology Applied Microbiology and Biotechnology Biochemistry chemistry.chemical_compound Enzyme Membrane Invertase chemistry Food science Inulinase
Zdroj:	Process Biochemistry. 37:863-868
ISSN:	1359-5113
DOI:	10.1016/s0032-9592(01)00283-7
Popis:	Growing cells of thermophilic Bacillus sp. 11, producer of a thermostable exo-inulinase, were immobilized on formaldehyde-activated polysulphone membranes. The biocatalysts obtained showed 1.5–2 fold higher enzyme yields (inulinase and invertase activities) than those of free cells. Active cell growth and no alterations of the membrane structure were observed by scanning electron microscopy (SEM) after 10 days (five cycles) repeated batch cultivation. The residual enzyme activities of the biocatalysts were 60–90% of their initial activities at the end of fifth run of semicontinuous cultivation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1317ea4b09aa2e5cb0d649f59c65decb https://doi.org/10.1016/s0032-9592(01)00283-7 Zobrazit plný text záznamu Full Text from ScienceDirect