Label-free photoelectrochemical immunoassay for CEA detection based on CdS sensitized WO3@BiOI heterostructure nanocomposite
| Autor: | Tong Zhang, Bin Xing, Qingzhi Han, Dan Wu, Malik Saddam Khan, Rongyu Wang, Qin Wei |
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| Rok vydání: | 2018 |
| Předmět: |
Materials science
Photoelectrochemistry Biomedical Engineering Biophysics Analytical chemistry 02 engineering and technology 010402 general chemistry 01 natural sciences Matrix (chemical analysis) Carcinoembryonic antigen Electrochemistry medicine Detection limit Reproducibility Nanocomposite biology medicine.diagnostic_test General Medicine 021001 nanoscience & nanotechnology 0104 chemical sciences Immunoassay Electrode biology.protein 0210 nano-technology Biotechnology Nuclear chemistry |
| Zdroj: | Biosensors and Bioelectronics. 99:493-499 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2017.08.034 |
| Popis: | In this work, a label-free photoelectrochemical (PEC) immunosensor for the detection of carcino embryonic antigen (CEA) was developed based on CdS nanowires (NWs) sensitized WO3@BiOI heterostructure. The construction procedure of ITO/WO3@BiOI@CdS electrode was done by dipping the WO3@BiOI modified indium-tin oxide (ITO) electrode interchangeably into [Cd(NH3)4]2+ and S2- solution for several times. Then the ITO/WO3@BiOI@CdS electrode was used as a matrix for the subsequent immobilization of CEA antibody (Ab). The obtained label-free PEC immunosensor showed an excellent PEC performance toward CEA detection. Under optimal conditions, the PEC immunosensor have a sensitive response to CEA in a liner range of 0.01ng/mL to 50ng/mL with a detection limit of 3.2pg/mL. The proposed PEC immunosensor exhibited good stability, high sensitivity as well as reproducibility and storage stability. Moreover, the obtained PEC immunosensor also showed a satisfied result in human serum sample analysis. |
| Databáze: | OpenAIRE |
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