Abstract 759: RNA Complete BCT: A novel blood collection tube targeting circulating RNA and extracellular vesicles
| Autor: | Nursen Binbuga, Eunju Seong, Lisa Bartron, Sean Salamifar, Nicholas M. George |
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| Rok vydání: | 2020 |
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| Zdroj: | Cancer Research. 80:759-759 |
| ISSN: | 1538-7445 0008-5472 |
| DOI: | 10.1158/1538-7445.am2020-759 |
| Popis: | Precision medicine, specifically in medical oncology, has been significantly enabled and the pace accelerated with the advent of liquid biopsy-based diagnostics. While liquid biopsy assays targeting circulating cell-free DNA (cfDNA) provide useful diagnostic information, such as mutational burden, they are limited to a static assessment of the disease state due to cfDNA derivation from dying cells. Assays exploiting circulating extracellular vesicles (EVs) and EV-associated cargo, such as cell-free RNA (cfRNA), provide a comprehensive and dynamic view of the disease as these analytes derive from both living and dying cells. A significant hurdle in making liquid biopsy testing with EVs and cfRNA commonplace is donor specimen integrity. Collected patient blood samples undergo time-dependent degradation leading to increases in disease non-specific EVs and cfRNA thereby compromising the accuracy and reproducibility of analyte measurement. To overcome this vulnerability we have developed the Streck RNA Complete BCT, a first of its kind blood collection tube intended for stabilizing draw-time concentrations of extracellular vesicles and cell-free RNA. Intended uses include direct analysis of EV populations and indirect analysis of cfRNA through RT-PCR or Next-generation Sequencing-based methods. Incorporation of this blood collection tube into the pre-analytical workflow will allow for delayed sample processing without effect on sample integrity or downstream analyte analysis. Citation Format: Nicholas M. George, Lisa Bartron, Nursen Binbuga, Sean Salamifar, Eunju Seong. RNA Complete BCT: A novel blood collection tube targeting circulating RNA and extracellular vesicles [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 759. |
| Databáze: | OpenAIRE |
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