Rutin improves glutamate uptake and inhibits glutamate excitotoxicity in rat brain slices
| Autor: | Jéssica Teles-Souza, Alessandra Bispo da Silva, Adriano Martimbianco de Assis, Rafael Short Ferreira, Diogo O. Souza, Victor Diogenes Amaral da Silva, Maria de Fátima Dias Costa, Silvia Lima Costa, Yanier Nuñez-Figueredo, Juliana Silva, Erica Patricia Lima Pereira, Fillipe M. de Araújo, Cleide dos Santos Souza, José Cláudio Fonseca Moreira, Yasmine Nonose |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Programmed cell death Chemistry Excitotoxicity Glutamate receptor General Medicine Pharmacology medicine.disease_cause Neuroprotection 03 medical and health sciences Rutin chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Cerebral cortex 030220 oncology & carcinogenesis Glutamine synthetase Genetics medicine Molecular Biology Astrocyte |
| Zdroj: | Molecular Biology Reports. 48:1475-1483 |
| ISSN: | 1573-4978 0301-4851 |
| DOI: | 10.1007/s11033-021-06145-y |
| Popis: | Rutin is an important flavonoid consumed in the daily diet. It is also known as vitamin P and has been extensively investigated due to its pharmacological properties. On the other hand, neuronal death induced by glutamate excitotoxicity is present in several diseases including neurodegenerative diseases. The neuroprotective properties of rutin have been under investigation, although its mechanism of action is still poorly understood. We hypothesized that the mechanisms of neuroprotection of rutin are associated with the increase in glutamate metabolism in astrocytes. This study aimed to evaluate the protective effects of rutin with a focus on the modulation of glutamate detoxification. We used brain organotypic cultures from post-natal Wistar rats (P7–P9) treated with rutin to evaluate neural cell protection and levels of proteins involved in the glutamate metabolism. Moreover, we used cerebral cortex slices from adult Wistar rats to evaluate glutamate uptake. We showed that rutin inhibited the cell death and loss of glutamine synthetase (GS) induced by glutamate that was associated with an increase in glutamate-aspartate transporter (GLAST) in brain organotypic cultures from post-natal Wistar rats. Additionally, it was observed that rutin increased the glutamate uptake in cerebral cortex slices from adult Wistar rats. We conclude that rutin is a neuroprotective agent that prevents glutamate excitotoxicity and thereof suggest that this effect involves the regulation of astrocytic metabolism. |
| Databáze: | OpenAIRE |
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