Description of Salinimonas profundi sp. nov., a deep-sea bacterium harboring a transposon Tn6333

Autor: Xiao-Mei Zhang, Ai-Hua Zhang, Jian-Ke Huang, Dao-Feng Zhang, Hua-Peng Xue, Xi-Wen Cui, Wen-Jun Li
Rok vydání: 2021
Předmět:
Zdroj: Antonie van Leeuwenhoek. 114:69-81
ISSN: 1572-9699
0003-6072
DOI: 10.1007/s10482-020-01501-7
Popis: A Gram-staining-negative bacterium, strain HHU 13199T, was isolated from a marine sediment sample collected from South China Sea (119°19.896’E, 19°41.569’N) at a depth of 2918 m. The 16S rRNA gene sequence analysis indicated that strain HHU 13199T represents a member of the genus Salinimonas with the highest sequence similarity (99.8%) to the type strain S. iocasae KX18D6T. However, the average nucleotide identity values and digital DNA–DNA hybridization between strain HHU 13199T and closely related members of the genus Salinimonas were all below the cut-off level (95–96 % and 70%, respectively) for species delineation. This strain grew with sea salt of 0.5–18% (w/v) (optimum, 2–5%), but no growth observed when using NaCl instead. The major fatty acids are C16:0, summed feature 3 (C16:1ω7c and/or C16:1ω6c), and summed feature 8 (C18:1ω7c and/or C18:1ω6c). The predominant isoprenoid quinone is ubiquinone-8. The polar lipids mainly consist of phosphatidylethanolamine, and phosphatidylglycerol. Genomic characterization revealed that strain HHU 13199T harbors a distinct type I-F CRISPR-Cas system and plenty of genes associated with heavy metal resistance, including a transposon (Tn6333) conferring mercury resistance. In addition, a phylogenetic tree based on the bac120 core genes suggested that the genus Salinimonas should be a subclade within Alteromonas. On the basis of the phenotypic, phylogenetic and chemotaxonomic characterizations, strain HHU 13199T represents a novel species of the genus Salinimonas, for which the name Salinimonas profundi sp. nov. is proposed. The type strain is HHU 13199T (= KCTC 72837T = MCCC 1K04127T).
Databáze: OpenAIRE
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