Coronary artery bypass grafting surgery differentially modulates the leucocyte gene expression profile
| Autor: | Alexis J Perros, Fenny Chong, Sanne Engkilde-Pedersen, Arlanna Esguerra-Lallen, Kelly Rooks, Helen M Faddy, Elise Hewlett, Rishendran Naidoo, John-Paul Tung, John F Fraser, Peter Tesar, Marc Ziegenfuss, Susan Smith, Donalee O’Brien, Robert L Flower, Melinda M Dean |
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| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 204:69.18-69.18 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.204.supp.69.18 |
| Popis: | Coronary artery bypass grafting (CABG) surgery triggers a systemic inflammatory response activating leucocyte subsets and cytokine secretion. Changes to the patient immune profile post-surgery may contribute to adverse outcomes such as infection. Investigating patient immune modulation at the molecular level may improve our understanding of the underlying mechanisms associated with adverse outcomes post-surgery. This study assessed changes to the leucocyte gene expression profile of CABG patients (n=50) at 5 time-points (admission, intra-operative, ICU, day 3, day 5). RNA was isolated from freshly collected whole blood for cDNA synthesis, pre-amplification and RT-PCR of 48 genes using the TaqMan Gene Expression Array Cards (Applied Biosystems). Gene expression at each time-point was compared to admission (repeated-measures one-way ANOVA with Dunnett’s post-test; P |
| Databáze: | OpenAIRE |
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