Popis: |
Inability of arbuscular mycorrhizal fungi to grow in pure cultures without living host roots is one of the most challenging hurdles to studying their symbiotic relationship with plants and their effect on cell growth and secondary metabolite production. In this regard, Piriformospora indica, an arbuscular mycorrhiza-like fungus, can be used as a very promising tool, due to its phytopromotional effect and ability to enhance production of desired phytochemicals. This chapter gives a detailed procedure for developing a co-culture system between plant cells of Linum album and fungal cells of P. indica in suspension cultures, for enhanced production of the anticancer drug podophyllotoxin. |