Capsular extracellular polysaccharide and hydroxamate formation by iron‐deficientazotobacter chroococcumB‐8

Autor:	Frank A. Fekete, Philip R. Lapp, Louis A. Profenno, Nicholas F. Ferrala, Lynn M. Nadeau, Gary A. Mabbott
Rok vydání:	1988
Předmět:	Siderophore biology Physiology Chemistry Auxotrophy Metabolism Carbohydrate biology.organism_classification medicine.disease_cause Microbiology Biochemistry Nitrogen fixation medicine Azotobacter chroococcum Diazotroph Agronomy and Crop Science Bacteria
Zdroj:	Journal of Plant Nutrition. 11:945-957
ISSN:	1532-4087 0190-4167
DOI:	10.1080/01904168809363859
Popis:	The free‐living diazotroph Azotobacter chroococcum B‐8 requires a high complement of Fe to support nitrogen fixation. The yield of capsular extracellular polysaccharide (EPS) and the formation of high‐affinity Fe‐binding compounds were used to measure the response of A. chroococcum B‐8 to iron‐stressed growth conditions. Cultures were nutritionally stressed for Fe by successive transfers on Fe‐deficient nitrogen‐free solid‐plating media, or on the same media supplemented with NO3‐ or NH4 + as the sources of fixed nitrogen. Under all nitrogen growth conditions, the same two anodally‐migrating Fe‐binding compounds were detected using the paper electrophoresis siderophore assay. Both Fe‐binding compounds were Csaky positive for bound hydroxylamines and both relieved the growth requirement of the hydroxamate auxotroph, Arthrobacter flavescens JG‐9. Fe‐deficient liquid cultures, grown in the presence of N2, NO3 ‐, or NH4 +, produced much higher levels of hydroxamate over the Fe‐sufficient control cult...
Databáze:	OpenAIRE
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