Purification and characterization of the extracellular and cell-bound lipases from a Penicillium cyclopium variety
Autor: | Louis-Claude Comeau, Najia El Abbadi, Danièle Druet |
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Rok vydání: | 1992 |
Předmět: | |
Zdroj: | Applied Microbiology and Biotechnology. 37:745-749 |
ISSN: | 1432-0614 0175-7598 |
DOI: | 10.1007/bf00174840 |
Popis: | A new strain of Penicillium cyclopium was found on mouldy Grenoble walnuts. From the culture filtrate, two new extracellular lipases (A- and B-lipase) were isolated and characterized. The mycelium contained one of the two forms of lipase (B-lipase). The specificity of the cell-bound lipase linked to the mycelium was then studied. The enzyme was specific to shortchain homogeneous triacylglycerols; it preferred to attack the o-position rather than the p-position, and showed stereospecificity on the sn-1 position. |
Databáze: | OpenAIRE |
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