Manganese Peroxidase-Based Electro-Oxidation of Bisphenol A at Hydrogellic Polyaniline-Titania Nanocomposite-Modified Glassy Carbon Electrode

Autor: Lindsay Wilson, Nomaphelo Ntshongontshi, Unathi Sidwaba, Tesfaye Waryo, Usisipho Feleni, Emmanuel I. Iwuoha
Rok vydání: 2019
Předmět:
Zdroj: Electrocatalysis. 10:323-331
ISSN: 1868-5994
1868-2529
Popis: In addition to attack by communicable diseases and unforeseen outbreak of different pathogens, endocrine-disrupting chemicals (EDCs) destroy the human system. Bisphenol A (BPA) is an endocrine-disrupting chemical found in daily useable plastic-ware and is linked to health concerns such as obesity, insulin resistance, cancer, and alteration in the expression levels of immunological response cytokines. This study reports an electrochemical manganese peroxidase biosensor fabricated on a glassy carbon electrode modified with a nanocomposite comprising conducting polyaniline (PANI), polymethyl methacrylate (PMMA), and titanium dioxide (TiO2) nanoparticles. Incorporation of PMMA and TiO2 into PANI was confirmed by Raman spectroscopy, ultraviolet-visible (UV-Vis) spectroscopy, and square wave voltammetry (SWV). The PANI composite served as an excellent receiving matrix for manganese peroxidase (MnP), an oxidoreductase well discovered for degradation of phenolic compounds. The biosensor showed excellent electrochemical degradation and oxidation of BPA, exhibiting a linear relationship between its current response and BPA concentrations within the range between 0.2 and 1.2 nM. The calculated detection limit of 0.17 nM signifies the excellent sensitivity and potential of the reported biosensor towards detection of BPA at trace amounts in environmental samples.
Databáze: OpenAIRE
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