Crystallization And Preliminary X-Ray Diffraction Study Of Riboflavin Synthase
| Autor: | Zdzislaw Wawrazak, Joseph C. Calabrese, Douglas B. Jordan, Paul V. Viitanen |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Diffraction
Complete data biology Resolution (electron density) Diglyme General Medicine medicine.disease_cause Biochemistry law.invention chemistry.chemical_compound Crystallography Riboflavin synthase chemistry Structural Biology law X-ray crystallography medicine biology.protein Crystallization Escherichia coli |
| Zdroj: | Protein & Peptide Letters. 8:69-73 |
| ISSN: | 0929-8665 |
| Popis: | Escherichia coli riboflavin synthase crystallizes at 22 C in the presence of 7-10percent by volume diglyme, 20-50 mM MgCl2 and pH 7.0. In this medium diffraction quality crystals are routinely obtained within 5 h and they are stable for 10 weeks. The crystals are orthogonal in space group P212121 with unit cell dimensions of a=52.4 A , b = 62.1 A, c = 218.8 A. A 97percent complete data set was collected at 2.1 A resolution. |
| Databáze: | OpenAIRE |
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