Screening of banana bunchy top virus through multiplex PCR approach

Autor:	Sivalingam Elayabalan, K. K. Kumar, P. Balasubramanian, K. Kalaiponmani, K. Angappan, A. Chandrasekar
Rok vydání:	2011
Předmět:	food and beverages RNA-dependent RNA polymerase Effective management Biology Coat protein biology.organism_classification Virology law.invention Banana bunchy top virus law Multiplex polymerase chain reaction Primer (molecular biology) Agronomy and Crop Science Polymerase chain reaction
Zdroj:	Archives Of Phytopathology And Plant Protection. 44:1920-1925
ISSN:	1477-2906 0323-5408
DOI:	10.1080/03235408.2010.522820
Popis:	Bunchy top disease caused by the banana bunchy top virus (BBTV) is a serious disease in hill banana. Detection of the BBTV infection in the planting material could help in the effective management of the disease. An attempt was made to develop a sensitive polymerase chain reaction (PCR) and multiplex PCR-based method for detection of BBTV in hill banana. DNA was isolated from the experimental plants at third and sixth months after planting. Multiplex PCR was done with Coat Protein (CP) and Replicase (Rep) gene-specific primer, and banana ethylene insensitive like protein (EISL) primer as internal control to identify failure in PCR reaction. This study revealed that multiplex PCR is effective for BBTV screening in hill banana with the advantage of overcoming the false positive in PCR amplification.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::12a119a8673a4b7deb67d1e924458870 https://doi.org/10.1080/03235408.2010.522820 Zobrazit plný text záznamu