A 3-Day Functional Unloading is Accompanied by an Increase in the TTN Gene Expression in the Rat Soleus Muscle without Changes in Alternative Splicing from Exon 50 to Exon 111
| Autor: | L. G. Bobyleva, T. L. Nemirovskaya, Ivan M. Vikhlyantsev, S. P. Belova, Yu. V. Gritsyna, V. K. Zhalimov, Boris Shenkman, A. D. Ulanova |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Soleus muscle Control level Messenger RNA 030102 biochemistry & molecular biology biology Chemistry Alternative splicing Biophysics musculoskeletal system Molecular biology 03 medical and health sciences Exon 030104 developmental biology Gene expression biology.protein Titin tissues |
| Zdroj: | Biophysics. 64:683-689 |
| ISSN: | 1555-6654 0006-3509 |
| DOI: | 10.1134/s0006350919050245 |
| Popis: | —Changes in the expression of the titin gene and alternative splicing of titin pre-mRNA from exon 50 to exon 111 in rat soleus muscle were analyzed following 3-day functional unloading (the HS group). Using real-time RT-PCR, it was found that the expression level of the titin gene in the rat soleus muscle from the HS group was higher by 1.81 times (p ≤ 0.01, n = 6) than the control level (n = 7). It was shown that all studied exons of titin mRNA are present in rats soleus muscle from the two groups. Our results demonstrate that 3-day functional unloading is accompanied by an increase in the expression of the titin gene in rat soleus without changes in alternative splicing from exon 50 to exon 111. |
| Databáze: | OpenAIRE |
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