Anti-Tn human monoclonal antibodies generated following active immunization with partially desialylated ovine submaxillary mucin

Autor:	Kevin P. O'Boyle, Kathryn E. Wright
Rok vydání:	1994
Předmět:	medicine.diagnostic_test biology medicine.drug_class Synthetic antigen Immunology Tn antigen Ovine Submaxillary Mucin General Medicine Monoclonal antibody Active immunization Molecular biology Immune system Immunoassay medicine biology.protein Immunology and Allergy Antibody
Zdroj:	Human Antibodies. 5:25-31
ISSN:	1875-869X 1093-2607
DOI:	10.3233/hab-1994-51-204
Popis:	Partially desialylated ovine submaxillary mucin plus an immunological adjuvant has been used by us to induce a humoral immune response to Tn antigen and sialylated Tn in colon cancer patients at risk of recurrence. Peripheral blood lymphocytes were purified from one of these patients with a high IgM titer reactive with Tn antigen transformed with Epstein-Barr virus and subsequently fused with a human-mouse heteromyeloma cell line, HMMA2.l1TGjO. Clones were screened and sub cloned using an enzyme-linked immunoassay andfour stable IgM-secreting clones were tested for reactivity with a variety of natural and synthetic antigens, paraffin-embedded colon carcinomas and normal colonic mucosa to determine their specificity. Four human IgM monoclonal antibodies reactive predominantly with Tn antigen were produced and shown to react with a mucinous human colon carcinoma cell line, LS-174T, and with paraffin-embedded human colon carcinomas. We conclude that modified ovine submaxillary mucin is an effective vaccine for generating a humoral immune response directed to Tn antigen present on human colon cancer. [Hum Antibod Hybridomas 1994; 5: 25-31]
Databáze:	OpenAIRE
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