In vitro and in vivo antileukemic activity of B43-pokeweed antiviral protein against radiation-resistant human B-cell precursor leukemia cells
| Autor: | Dorothea E. Myers, Mridula Chandan-Langlie, Kevin G. Waddick, Lisa M. Chelstrom, Nilgun Tumer, Fatih M. Uckun, J. D. Irvin, Roland Gunther |
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| Rok vydání: | 1995 |
| Předmět: |
Severe combined immunodeficiency
medicine.medical_treatment Immunology Antiviral protein Cell Biology Hematology Immunotherapy Biology Total body irradiation medicine.disease Biochemistry Virology Transplantation Leukemia Immunotoxin hemic and lymphatic diseases medicine Cancer research Progenitor cell |
| Zdroj: | Blood. 86:4228-4233 |
| ISSN: | 1528-0020 0006-4971 |
| Popis: | B-cell precursor (BCP) leukemia is the most common form of childhood cancer and represents one of the most radiation-resistant forms of human malignancy. In this study, we examined the antileukemic efficacy of the B43 (anti-CD19)-pokeweed antiviral protein (B43-PAP) immunotoxin against radiation-resistant BCP leukemia cells. B43-PAP caused apoptosis of radiation-resistant primary BCP leukemia cells, killed greater than 99% of radiation-resistant primary leukemic progenitor cells from BCP leukemia patients, and conferred extended survival to severe combined immunodeficiency (SCID) mice xenografted with radiation- resistant human BCP leukemia. Furthermore, the combination of B43-PAP and total body irradiation (TBI) was more effective than TBI alone in two SCID mouse bone marrow transplantation models of radiation- resistant human BCP leukemia. Thus, B43-PAP may prove useful in the treatment of radiation-resistant BCP leukemia. |
| Databáze: | OpenAIRE |
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