An effective ICT-based and ratiometric fluorescent probe for sensing sulfite
| Autor: | Xiang-Jian Cao, Jin-Ting Liu, Li-Jie Zhang, Zhao-Yang Wang, Bao-Xiang Zhao |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Inorganic chemistry
02 engineering and technology 010402 general chemistry 01 natural sciences chemistry.chemical_compound Sulfite Materials Chemistry Electrical and Electronic Engineering Instrumentation chemistry.chemical_classification Detection limit Biomolecule Metals and Alloys Glutathione 021001 nanoscience & nanotechnology Condensed Matter Physics Fluorescence Combinatorial chemistry 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials chemistry Proton NMR 0210 nano-technology Selectivity Cysteine |
| Zdroj: | Sensors and Actuators B: Chemical. 236:741-748 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2016.06.055 |
| Popis: | We have developed a ratiometric fluorescent probe based on ICT mechanism for imaging intracellular sulfite (SO 3 2− ) in HeLa cells. The probe could selectively stain cells with scarce cytotoxicity and excellent photostability. The probe displayed a high selectivity for sulfite over other anions and active small biological molecules including cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) at room temperature. The reaction could complete within 30 s and detection limit was as low as 76 nM, which is superior to some reported probes. The detecting mechanism was proved by MS, 1 H NMR and theoretical calculation. The probe can also detect endogenous sulfite in HepG2 cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect