Use of Tn phoA to tag exported proteins in Myxococcus xanthus

Autor:	Isabelle Buon, Annick Breton, Janine F. Guespin-Michel
Rok vydání:	1990
Předmět:	Signal peptide fungi Mutant Phosphatase Biology biology.organism_classification Microbiology Molecular biology Secretory protein Genetics Extracellular bacteria Alkaline phosphatase Myxococcaceae Myxococcus xanthus Molecular Biology
Zdroj:	FEMS Microbiology Letters. 67:179-186
ISSN:	0378-1097
DOI:	10.1111/j.1574-6968.1990.tb13859.x
Popis:	Tn phoA was introduced into Myxococcus xanthus, and insertion mutants that expressed the E. coli alkaline phosphatase (PhoA) were selected. These mutants displayed hybrid proteins of various sizes containing PhoA antigenicity. The transposed strains expressed different levels of E. coli alkaline phosphatase activity; in addition they varied in the fraction of extracellular enzyme from mutants with mostly cell bound activity to mutants that produce the phosphatase in the extracellular medium. Therefore sequences coding for signal peptides should exist in M. xanthus, and Tn phoA can be a suitable tool to study the events involved in protein secretion in M. xanthus.
Databáze:	OpenAIRE
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