| Autor: |
B. Porsch, M. Jirsa, P. Kotal, V. Kordač |
| Rok vydání: |
1985 |
| Předmět: |
|
| Zdroj: |
Journal of Chromatography A. 333:141-151 |
| ISSN: |
0021-9673 |
| DOI: |
10.1016/s0021-9673(01)87332-5 |
| Popis: |
A simple isocratic procedure for the separation of porphyrin esters according to the number of carboxyl groups using an aminopropyl-bonded silica column and a simple binary eluent is suggested. In this way, problems with the choice of a suitable unmodified silica column for isocratic separation are circumvented; entirely different aminopropyl-bonded silicas of various origins exhibit almost identical behaviour. The influence of end-capping of residual silanols and the chemical substitution of the primary amino group on the separation was studied. To achieve an isocratic separation of porphyrin esters on the bare silica used, the concentration of surface silanol groups must be decreased; suitable concentrations are tailored by means of partial trimethylsilylation. The isocratic separation of porphyrin esters can also be achieved on cyanopropyl-bonded silica. The suggested optimum procedure, using primary amino-bonded silica, gives the highest resolution and allows the baseline separation of porphyrin esters with two to eight carboxyl groups together with their Cu 2+ complexes and synthetic tetraphenylporphyrin as the internal standard. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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