Extraction protocol and mass spectrometry method for quantification of doxorubicin released locally from prodrugs in tumor tissue

Autor:	Yongxuan Su, Stephen P. Adams, Eran Zahavy, Sadik C. Esener, Wolf Wrasidlo, John T. Norton, Tomoko Hayashi, Stuart Ibsen
Rok vydání:	2013
Předmět:	Detection limit Biodistribution Chromatography Chemistry Extraction (chemistry) medicine Doxorubicin Prodrug Mass spectrometry Tandem mass spectrometry Spectroscopy medicine.drug Epirubicin
Zdroj:	Journal of Mass Spectrometry. 48:768-773
ISSN:	1076-5174
DOI:	10.1002/jms.3221
Popis:	The localized conversion of inactive doxorubicin prodrug chemotherapeutics to pharmacalogically active forms is difficult to quantify in mouse tumor models because it occurs only in small regions of tissue. The tumor tissue extraction protocol and LC-MS/MS analysis method described here were optimized to obtain a detection limit of 7.8 pg for the activated doxorubicin and 0.36 ng for the doxorubicin prodrug. This method can be useful for determining the biodistribution and activation efficiency for many different doxorubicin prodrugs. It can also be used for quantification of doxorubicin from tumor models that have poor vascularization resulting in low tissue accumulation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::12600282ad837821381cade0fc5c3eb6 https://doi.org/10.1002/jms.3221 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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