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Genomic in situ hybridization (GISH) allows genomes differing in molecular composition to be discriminated visually in cell nuclei. Total genomic DNA from a given genotype (e.g. AA) is used to probe for chromosomes of A in hybrids (e.g. AB; Plate 1 b, c), allopolyploids (e.g. AABB; Bennett et al. 1992; Kenton et al. 1993b; Leggett and Markhand 1995), or lines of B introgressed with DNA from A (Mukai et al. 1993a; Parokonny and Kenton 1995). The hybridization target is chromosomal DNA in spreads (Plate 1 b, c), sections (Leitch et al. 1990) or whole, isolated nuclei (van Dekken et al. 1989). The last two examples are useful for studying genome domains in hybrid nuclei, a major application of GISH (Schwarzacher et al. 1989; Leitch et al. 1990). |
