Broth recycling in high carbon demanding single cell oil fermentation increased the product to effluent generation ratio
| Autor: | Pratima Gupta, Batul Diwan |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Chemistry food and beverages Substrate (chemistry) chemistry.chemical_element Bioengineering Pulp and paper industry 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Yeast Hydrolysate Dilution High carbon 03 medical and health sciences 030104 developmental biology 010608 biotechnology Fermentation Carbon Effluent |
| Zdroj: | Process Biochemistry. 75:68-73 |
| ISSN: | 1359-5113 |
| Popis: | In the attempt to minimize the incessant rise in industrial effluent discharge and maximize the existing resource utilization, present work demonstrates a proof of concept study on recycling of Single cell oil (SCO) fermentation effluent. To offset the high production cost associated with SCO fermentation, saccharified rice straw was utilized as cheap fermentable carbon (C) source. Fermentation broth formulated from Non-detoxified Liquid hydrolysate (NDLH) of rice straw(C: 50 g/l), was recycled without any dilution and carbon supplementation (mimicking a fermentation effluent) for two subsequent rounds to culture indigenous oleaginous yeast isolates NBT-8 and NBT-13. The resultant lipid titers in three consecutive fermentations were 1.036 g/l, 0.546 g/l, and 0.34 g/l from NBT-13 while 1.2 g/l, 0.96 g/l and 0.57 g/l from NBT-8 respectively. It was noteworthy that even at 100% recycle ratio, the NDLH based broth served as effective inexpensive substrate for NBT-8 up to two subsequent cycles resulting in good cumulative lipid titer of 2.16 g/l. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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