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Plant-derived compounds such as essential oils (EOs) are sources of protection for stored products. In some cases, inactive ingredients that co-occur with EOs may render them more toxic or less desirable. Thus, there has been a shift away from extract-based crude mixtures to single repellent substances. The repellency activity of the capsaicin agonist, vanillyl butyl ether (VBE), which is a thermal-sense compound affecting the transient receptor potential vanilloid (TRPV) 1 channel of mammals, was investigated for control of the red flour beetle, Tribolium castaneum. The repellency activity of VBE was dose-dependent and almost comparative to DEET in an area preference test. To clarify the mode of action, RNA interference (RNAi) was used for transcriptional knockdown of two TRPV channels, Nanchung (TcNan) or Inactive (TcIav). Reduction of each of the transcript levels led to a significant decrease in VBE repellency activity, but no significant decrease in DEET repellency. These results suggested that the repellency activity of VBE was mediated by the TRPV channels, and capsaicin agonists might serve as an alternative to EOs for the protection of stored products. |
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