Immunological markers in the diagnosis of inflammatory bowel diseases in children
| Autor: | А.А. Stepanova, О.N. Varlamova, E.A. Dementyeva, O.P. Gurina, A.I. Khavkin, А.Е. Blinov |
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| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Voprosy dietologii. 10:5-10 |
| ISSN: | 2414-9004 2224-5448 |
| Popis: | Objective. To study the specificities of autoimmune response in children suffering from Crohn's disease and interrelationships between autoagression parameters. Patients and methods. We examined 62 patients aged 2–17 years with a confirmed diagnosis of Crohn’s disease. The examination included flow cytometric immunophenotyping of lymphocytes, examination of serum concentrations of total and specific immunoglobulins IgE against allergens of the fungi Saccharomyces cerevisiae and Candida albicans (enzyme-linked immunosorbent assay – ELISA), circulating immune complexes (CICs), concentrations of autoantibodies (AB) against S. cerevisiae (ASCA) IgA and IgG, anti-neutrophil cytoplasmic AB (ANCA MPO, ANCA PR3), antinuclear AB (ANAs), AB against DNAds, DNAss, autoantibodies against antigens of the small and large intestines, and the pancreas (ELISA). Results. Activation of helper T cells, decreased NK cell levels, higher levels of γδ-Т cells were detected. Specific IgЕ against к S. cerevisiae could be noted in 70.9% of patients. In 35.5% of cases, higher levels of ASCA (IgA, IgG) were found. Blood concentrations of AB against DNAds, DNAss exceeded the norm in 4.8 and 16.1% of patients, respectively. Higher levels of CICs were found in 20 (32.3%) of patients. ANAs concentrations corresponded to the norm. Strong positive correlations between many parameters of autoimmune response are indicative of the intensity of the immunopathological process in CD. Conclusion. Immunological diagnosis in CD is necessary for assessing the severity of disease, for optimization of differential diagnosis, establishment of a prognosis and selection of individual therapy for regulation of immune responses. Key words: autoantibodies, Crohn’s disease, immunodiagnosis, immunophenotyping of lymphocytes |
| Databáze: | OpenAIRE |
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