Regioselective Biotransformation of Phloretin Using Streptomyces avermitilis MA4680
| Autor: | June-Hyung Kim, Kwon-Young Choi, Woo-Il Kim, Jong-Ki Lee, Byung-Gee Kim |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0303 health sciences biology Stereochemistry Phloretin technology industry and agriculture Biomedical Engineering Bioengineering Dihydrochalcone Metabolism BSTFA biology.organism_classification 01 natural sciences Applied Microbiology and Biotechnology Hydroxylation 03 medical and health sciences chemistry.chemical_compound chemistry Biotransformation 010608 biotechnology Derivatization Streptomyces avermitilis 030304 developmental biology Biotechnology |
| Zdroj: | Biotechnology and Bioprocess Engineering. 25:272-278 |
| ISSN: | 1976-3816 1226-8372 |
| Popis: | Streptomyces avermitilis MA4680 was used for the biotransformation of phloretin, one of the dihydrochalcone found in apple bark. After LC analysis, two main biotransformed products were identified and they were further analyzed using GC/MS. After BSTFA derivatization of biotransformed product, they were interpreted as regioselectively hydroxylated products of phloretin in Bring. Maximum conversion of phloretin was 6.7% with 1 h of reaction, and the phloretin and reaction products were completely metabolized after 3 h of reaction due to cellular metabolism. Addition of 0.5 mM quinidine completely blocked hydroxylation of phloretin, which means the hydroxylation proceed by P450 monoxygenase dependent metabolism. Addition of Brij 35 detergent resulted in 150% increase of hydroxylated product due to facilitating transport of phloretin and its reaction product across cellular membrane. |
| Databáze: | OpenAIRE |
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