Hyperglycemia Decreases the Expression of ATP Synthase β Subunit and Enolase 2 in Glomerular Epithelial Cells
| Autor: | Sang Hoon Kim, O-Deuk Kwon, Gi-Yeon Han, Byoung-Geun Kim, Pan-Kyeom Kim, Patrick TaeJoon Hwang, Chan-Wha Kim, Young-Woo Jang, Hyun Jung Kim |
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| Rok vydání: | 2013 |
| Předmět: |
chemistry.chemical_classification
medicine.medical_specialty Reactive oxygen species ATP synthase biology Chemistry General Medicine medicine.disease General Biochemistry Genetics and Molecular Biology Muscle hypertrophy Diabetic nephropathy Pathogenesis Blot Enolase 2 Endocrinology Internal medicine medicine biology.protein Glycolysis |
| Zdroj: | The Tohoku Journal of Experimental Medicine. 231:45-56 |
| ISSN: | 1349-3329 0040-8727 |
| DOI: | 10.1620/tjem.231.45 |
| Popis: | Glomerular epithelial cells (GECs) are known to play a key role in maintaining the structure and function of the glomerulus. GEC injury induced by hyperglycemia is present in early-stage diabetic nephropathy (DN), which is the most common cause of renal failure. In an attempt to identify target proteins involved in the pathogenesis of GEC injury at early DN, we performed the proteomic analysis using primary cultures of GECs, prepared from the dissected rat glomeruli. The protein expression profiles in the two-dimensional electrophoresis gels were compared between GECs treated for three days with normal glucose (5 mM) and those with high glucose (30 mM) concentrations. These concentrations correspond to blood glucose concentrations under normoglycemia and hyperglycemia, respectively. Proteins with differential expression levels were identified using ESI-Q-TOF tandem mass spectrometry. The primary GECs cultured in hyperglycemic conditions showed cellular hypertrophy and increased production of reactive oxygen species, both of which reflect the GEC injury. Our proteomic analysis identified eight proteins with differential expression profiles, depending on glucose concentrations. Among them, we selected ATP synthase β subunit and enolase 2 that are related to energy metabolism and are down-regulated under hyperglycemia, and confirmed that hyperglycemia decreased the expression levels of ATP synthase β subunit and enolase 2 proteins by western blotting analysis. Hyperglycemia may impair mitochondrial function and alter glycolysis in GECs by down-regulating the expression of ATP synthase β subunit and enolase 2. The present study may provide a better understanding of the pathogenic mechanisms of GEC injury in early DN. |
| Databáze: | OpenAIRE |
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