3,3′-Diindolylmethane Enhances Glucose Uptake Through Activation of Insulin Signaling in 3T3-L1 Adipocytes
| Autor: | Hwan-Soo Yoo, Kyeong-Mi Choi |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
3 3'-Diindolylmethane medicine.medical_specialty Nutrition and Dietetics genetic structures biology Endocrinology Diabetes and Metabolism Glucose uptake Insulin medicine.medical_treatment Glucose transporter Medicine (miscellaneous) Diindolylmethane 03 medical and health sciences chemistry.chemical_compound Insulin receptor 030104 developmental biology Endocrinology chemistry Downregulation and upregulation Adipogenesis Internal medicine medicine biology.protein |
| Zdroj: | Obesity. 26:1153-1160 |
| ISSN: | 1930-7381 |
| DOI: | 10.1002/oby.22145 |
| Popis: | Objective Indole-3-carbinol (I3C), a naturally occurring compound found in cruciferous vegetables, and its metabolite 3,3'-diindolylmethane (DIM) reduce body mass and serum glucose levels in high-fat-diet-induced obese mice. This study aimed to determine whether I3C or DIM could increase glucose uptake via enhanced insulin sensitivity in 3T3-L1 adipocytes, as well as the mechanism involved. Methods 3T3-L1 preadipocytes were differentiated by using a mixture of adipogenic inducers, including a suboptimal concentration of insulin. Results DIM, but not I3C, increased adipocyte differentiation through upregulation of peroxisome proliferator-activated receptor γ and CCAAT/enhancer-binding protein α. DIM also enhanced glucose uptake by increasing expression of glucose transporter 4 in adipocytes. This was associated with DIM-enhanced phosphorylation of the signaling intermediates Akt, insulin receptor substrate-1, and insulin receptor early in differentiation. Conclusions Our findings suggest that DIM may improve insulin sensitivity through the activation of the insulin signaling pathway, leading to enhanced glucose uptake. |
| Databáze: | OpenAIRE |
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