Genetic Code Expansion in Enteric Bacterial Pathogens
| Autor: | Shixian Lin, Peng Chen, Huangtao Zheng |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Pyrrolysine Protein engineering Computational biology Biology Genetic code biology.organism_classification Enterobacteriaceae Yeast Amino acid 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Plasmid chemistry Bacteria |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493975730 |
| Popis: | The genetic code expansion strategy has become an elegant method for site-specific incorporation of noncanonical amino acids with diverse functionalities into proteins of interest in bacteria, yeast, mammalian cells, and even animals. This technique allows precise labeling as well as manipulation of a given protein to dissect its physiological and/or pathological roles under living conditions. Here, we demonstrate the extension of a recently emerged pyrrolysine-based genetic code expansion strategy for encoding noncanonical amino acids into enteric bacterial pathogens. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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