A noninvasive, direct real-time PCR method for sex determination in multiple avian species

Autor:	Jessica L. Brubaker, Paula F. P. Henry, Yu Chen, Daniel T. Sprague, Natalie K. Karouna-Renier, Kathryn Jenko
Rok vydání:	2010
Předmět:	Gel electrophoresis Chromatography Noninvasive sampling Biology DNA extraction Molecular biology law.invention Real-time polymerase chain reaction law Genetics Sample collection Ecology Evolution Behavior and Systematics Polymerase chain reaction Biotechnology
Zdroj:	Molecular Ecology Resources. 11:415-417
ISSN:	1755-098X
Popis:	Polymerase chain reaction (PCR)-based methods to determine the sex of birds are well established and have seen few modifications since they were first introduced in the 1990s. Although these methods allowed for sex determination in species that were previously difficult to analyse, they were not conducive to high-throughput analysis because of the laboriousness of DNA extraction and gel electrophoresis. We developed a high-throughput real-time PCR-based method for analysis of sex in birds, which uses noninvasive sample collection and avoids DNA extraction and gel electrophoresis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::11f1df83ca0ca917533abe6d13ad01ac https://doi.org/10.1111/j.1755-0998.2010.02951.x Zobrazit plný text záznamu Plný text