| Popis: |
Fungi often adapt to environmental stress by altering their size, shape, or rate of cell division. These morphological changes require reorganization of the cell wall, a structural feature external to the cell membrane composed of highly interconnected polysaccharides and glycoproteins. Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes that are typically secreted into the extracellular space to catalyze initial oxidative steps in the degradation of complex biopolymers such as chitin and cellulose. However, their roles in modifying endogenous microbial carbohydrates are poorly characterized. TheCEL1gene in the human fungal pathogenCryptococcus neoformans(Cn) is predicted by sequence homology to encode an LPMO of the AA9 enzyme family. TheCEL1gene is induced by host physiological pH and temperature, and it is primarily localized to the fungal cell wall. Targeted mutation of theCEL1gene revealed that it is required for the expression of stress response phenotypes, including thermotolerance, cell wall integrity, and efficient cell cycle progression. Accordingly, acel1Δdeletion mutant was avirulent in two models ofC. neoformansinfection. Therefore, in contrast to LPMO activity in other microorganisms that primarily targets exogenous polysaccharides, these data suggest thatCnCel1 promotes intrinsic fungal cell wall remodeling events required for efficient adaptation to the host environment.Author summaryFungi need to adapt quickly to environmental stresses to thrive. The fungal cell wall, which supplies support and integrity to the cell, is an essential compartment to react and interact with the surrounding environment. Rapid changes within the carbohydrate composition and architecture occur in response to environmental stresses. Lytic polysaccharide monooxygenases (LPMOs) are mononuclear copper-enzymes, secreted by microbes to assist in the first steps of remodeling and degrading complex and recalcitrant carbohydrates. In this study we explore the role of the putative AA9 family LPMOCnCel1 for growth and virulence of the fungal human pathogenCryptococcus neoformans. TheCEL1gene is highly up-regulated in presence of host stresses and acel1Δmutant strain is avirulent in a murine model of infection. Downstream analysis of virulence-associated phenotypes identified theCEL1gene to be required for thermotolerance as well as cell wall integrity, and efficient cell cycle progression in presence of host-mimicking stresses. Based upon those findings we propose thatCnCel1 likely promotes intrinsic fungal cell wall remodeling events essential for adaptation to the host environment. |
