Molecular and physiological characterization of Italian isolates of Pyrenochaeta lycopersici

Autor:	A. Brunetti, A. Porta-Puglia, Anna Oliva, Alessandro Infantino, Ernesto Lahoz, Maria Aragona
Rok vydání:	2003
Předmět:	biology Plant Science Fungi imperfecti Fungus biology.organism_classification Diagnostic tools Esterase Pyrenochaeta lycopersici RAPD Horticulture Botany Genetics Genetic variability Ecology Evolution Behavior and Systematics Solanaceae Biotechnology
Zdroj:	Mycological Research. 107:707-716
ISSN:	0953-7562
DOI:	10.1017/s0953756203007962
Popis:	Corky root of tomato caused by Pyrenochaeta lycopersici is a disease of concern in Italy and for many tomato growing areas in the world. Isolates of the fungus were characterized at both the physiological and molecular level. The optimal in vitro growth temperature for all isolates was 23 °C. All Italian isolates of P. lycopersici showed similar RAPD and esterase banding patterns. No relevant polymorphisms were detected after enzymatic digestion of PCR-amplified ITS and IGS regions. The overall results indicate a low degree of genetic variability within a collection of 43 Italian isolates. These data are of interest in breeding programs for resistance against corky root of tomato and they provide useful information for the development of molecular diagnostic tools for the rapid identification and detection of P. lycopersici.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::11be5aaec440f32d635c0b4a2767e7dc https://doi.org/10.1017/s0953756203007962 Zobrazit plný text záznamu Full Text from ScienceDirect