| Popis: |
The implementation of the T-ARMS PCR method in the detection of single nucleotide polymorphisms (SNPs) in the LEPR gene in chicken DNA samples has never been conducted. This research aims to design a specific protocol for exon 9 LEPR gene SNPs detection and detect LEPR gene expression or LEPR SNPs in Pelung chicken samples, F1Pelung, Layer, Broiler Cobb 500, F1Kambro chicken and F2Kambro chicken using the T-ARMS PCR method. Determination of LEPR gene correlation degree on Body Weight (BT) and Egg Productivity (PT) in F1 Kambro population and F2Kambro. Qualitative phenotype parameters showed six groups of segregated phenotypes compared to F1Kambro chicken. Growth of F2Kambro chicken weight reached 753.36 ± 155.31 grams in 8 weeks was not significant for F1Kambro chicken due to inbreeding depression (Fx = 25%, IR = 4.925%) and transversion of A LEPR allele mutations. Specific protocol detection of exon 9 LEPR gene SNPs using the T-ARMS PCR method can detect C127A LEPR mutations with IP: OP ratio 10:1 pmol / µM, chicken DNA template concentration of 100 ng / µL with annealing temperature of 55.7° C / 30s. The transversion mutation of C127A of LEPR exon 9 SNP were detected in DNA samples of F1Kambro hens (80%), F2Kambro roosters (20%), Broiler Cobb 500 hens (75%). The mutations were not detected in Layer, Pelung Blirik Hitam chicken and F1Pelung populations. |
