Validation of an Immunoassay for Detection and Quantitation of a Genetically Modified Soybean in Food and Food Fractions Using Reference Materials: Interlaboratory Study

Autor: James W Stave, Elke Anklam, Markus Lipp
Rok vydání: 2000
Předmět:
Zdroj: Journal of AOAC INTERNATIONAL. 83:919-928
ISSN: 1944-7922
1060-3271
DOI: 10.1093/jaoac/83.4.919
Popis: An immunoassay for detection of a specific genetically modified soybean (Roundup-Ready®) was validated on dried soybean powder in an interlaboratory study. Different percentages of genetically modified soybeans in nonmodified soybean matrix were evaluated in a blind study. Thirty-eight laboratories from 13 countries participated. The immunoassay was evaluated for 2 endpoints: (1) To give a semiquantitative result, i.e., determination of a given sample above or below a given threshold, or (2) to compute a quantitative result, i.e., percentage of genetically modified soybeans in the sample. Semiquantitative results showed that a given sample which contained
Databáze: OpenAIRE