A novel antioxidant rich compound 2-hydoxy 4-methylbenzaldehyde from Decalepis arayalpathra induces apoptosis in breast cancer cells
| Autor: | Ramar Thangam, Veeraperumal Suresh, Sivaraman Gokul, Srinivasan Sivasubramanian, Malairaj Sathuvan |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
chemistry.chemical_classification Reactive oxygen species Antioxidant Chemistry medicine.medical_treatment Cell Bioengineering 01 natural sciences Applied Microbiology and Biotechnology In vitro medicine.anatomical_structure Biochemistry Apoptosis 010608 biotechnology medicine Inner mitochondrial membrane Cytotoxicity Decalepis arayalpathra Agronomy and Crop Science 010606 plant biology & botany Food Science Biotechnology |
| Zdroj: | Biocatalysis and Agricultural Biotechnology. 21:101339 |
| ISSN: | 1878-8181 |
| DOI: | 10.1016/j.bcab.2019.101339 |
| Popis: | Decalepis arayalpathra is an endangered and endemic medicinal plant, mainly used for its aromatic and ethno-medical properties. In this study, we reported the biomedical potentials of 2-hydoxy 4-methylbenzaldehyde (2H4MB) from D. arayalpathra. The compound 2H4MB was isolated from fleshy root barks of the plants using steam-hydro distillation extraction method and quantified using HPLC analysis, which yielded 6.5 ± 0.8 μg g−1 of purified compound. Further, the antioxidant and anticancer effects in vitro were studied in detail using MDA-MB-231 cells by cell-based assays. 2H4MB was observed to display the features such as generation of reactive oxygen species (ROS), improved cytotoxicity, loss of mitochondrial membrane (Δψm) potential, and nuclear DNA fragmentations, leading to induce cellular apoptosis and cell cycle arrest. Further, the bioavailability of 2H4MB may facilitate its use as a potential cancer therapeutic agent. |
| Databáze: | OpenAIRE |
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