In vitro morphogenetic responses and comparative analysis of phthalides in the highly valued medicinal plant Ligusticum porteri

Autor:	D Goldhaber-Pasillas, Rachel Mata, Robert Bye, V Chávez-Ávila
Rok vydání:	2012
Předmět:	Pharmacology biology Callus formation Organic Chemistry Pharmaceutical Science Petroselinum crispum biology.organism_classification In vitro Analytical Chemistry Complementary and alternative medicine Germination Drug Discovery Botany Molecular Medicine Ligusticum porteri Explant culture
Zdroj:	Planta Medica. 78
ISSN:	1439-0221 0032-0943
DOI:	10.1055/s-0032-1320841
Popis:	The morphogenetic response of Ligusticum porteri was investigated as a part of a conservation strategy and was compared to that of Petroselinum crispum. After in vitro germination of seeds, plantlets were excised into different explants and cultured in an induction medium supplemented with 2,4-dichlorophenoxyacetic acid (0–18.09µM) or α-naphthaleneacetic (0–21.48µM) acid in combination with 6-benzylaminopurine (0–13.31µM) to induce callus formation. GC-MS analysis of calli, mature aerial parts and roots extracts of both species led to the identification of 3-butylidenephthalide (1), 3-n-butylphthalide (2) and 3,6,7,-trimethoxy-isobenzofuran-13(H)-one (3). This is the first report on phthalides production from in vitro cultures of L. porteri.
Databáze:	OpenAIRE
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