| Popis: |
Background: The aim of this study was to elucidate the features of expression of the inducer of expression of matrix metalloproteinases – EMMPRIN (EMN) and matrix metalloproteinase - MMP-1 in cell lines transformed with HPV16 and HPV18, and clinical samples of squamous cell carcinoma of the cervix uteri (SCC), as factors of tumor invasion.Material and methods: The study was carried out using RT-PCR and immunohistochemical studies (IHC).Results: The data obtained indicate that the expression of mRNA EMN and MMP-1 occurs in all cell lines. No direct correlation was found between the expression of EMN and MMP-1. Expression of mRNA of both EMN and MMP-1 in the HPV18 cell lines was more pronounced than in HPV16. The expression of MMP-1 in tumor tissue of SCC, as a rule, was significantly higher than in normal tissue, according to densitometry data by 5-7 times. The high MMP-1 mRNA expression was found in 90% of tumor samples. Expression of EMN mRNA was found in tumor and normal tissues. In most tumor samples, expression was at or below normal tissue level, and an increase in expression was detected in only isolated cases of SCC. Conclusion: The obtained data are important for understanding the process of tumor development and may have prognostic value. MMP-1 can serve as a marker of the invasive potential of SCC. |
