Studies on plant gums. Role of calcium in polysaccharide-protein interaction in the neem (Azadirachta indica) gum

Autor:	T. N. Pattabiraman, B. Ramakrishna Nayak, B. C. Shenoy
Rok vydání:	1979
Předmět:	chemistry.chemical_classification Chromatography biology Chemistry Plant Gums chemistry.chemical_element General Medicine Carbohydrate Calcium Azadirachta biology.organism_classification Polysaccharide General Biochemistry Genetics and Molecular Biology General Agricultural and Biological Sciences
Zdroj:	Journal of Biosciences. 1:61-68
ISSN:	0973-7138 0250-5991
DOI:	10.1007/bf02702887
Popis:	The partial removal of tightly bound Ca2+ from dialysed neem (Azadirachta indica) gum, resulted in the release of a basic protein from a highly anionic polysaccharide-protein complex as evidenced by chromatographic studies on TEAE-cellulose. Complete removal of Ca2+ caused, in addition, the release of a minor heteropolysaccharide which was found in association with the basic protein. These processes were reversed on the addition of Ca2+. The gum, in addition, contained a protein-rich component accounting for 35% protein and 7.5% total carbohydrate. This component behaved as a distinct entity during ion-exchange chromatography of the native gum solutions, or which were either partially or completely depleted of bound Ca2+.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::118a5f0fc7222959d1b5190c77509d1c https://doi.org/10.1007/bf02702887 Zobrazit plný text záznamu Full text from SpringerLink