The effect of wheat germ agglutinin on dinitrogen fixation, glutamine synthetase activity and ammonia excretion inAzospirillum brasilenseSp 245

Autor:	Mikhail A. Galkin, L. P. Antonyuk, Vladimir V. Ignatov, Oksana R. Fomina
Rok vydání:	1993
Předmět:	biology Nitrogenase Lectin Azospirillum brasilense biology.organism_classification Microbiology Wheat germ agglutinin chemistry.chemical_compound Agglutinin chemistry Biochemistry Glucosamine Glutamine synthetase Genetics biology.protein Bovine serum albumin Molecular Biology
Zdroj:	FEMS Microbiology Letters. 110:285-289
ISSN:	1574-6968 0378-1097
DOI:	10.1111/j.1574-6968.1993.tb06336.x
Popis:	Wheat germ agglutinin was found to increase glutamine synthetase and nitrogenase activities and excretion of N 2 -fixation product (NH 4 + ) in Azospirillum brasilense Sp 245. Each effect had a similar pattern and correlated well with each other. They were dynamic, had maxima in the middle of the exponential phase of growth, and were due to N- acetyl- D -glucosamine specificity of wheat germ agglutinin, since its preincubation with 10% N- acetyl- D -glucosamine caused the disappearance of the effects. When wheat germ agglutinin was replaced with Ulex europaeus agglutinin II possessing the same carbohydrate binding specificity all the above effects remained, replacing wheat germ agglutinin with concanavalin A with a different sugar specificity, or with bovine serum albumin led to their disappearance.
Databáze:	OpenAIRE
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