Characterisation by restriction endonuclease analysis and plasmid profiling ofVibrio ordaliistrains from salmon(oncorhynchus tshawytschaandoncorhynchus nerka)with vibriosis in new zealand

Autor:	Geoffrey W. de Lisle, B.J. Wards, Hansa H. Patel, Colin D. Anderson
Rok vydání:	1991
Předmět:	Transposable element Vibrio anguillarum Ecology biology Aquatic Science biology.organism_classification Microbiology Restriction enzyme Plasmid Vibrionaceae Oncorhynchus Vibrio ordalii Ecology Evolution Behavior and Systematics Salmonidae Water Science and Technology
Zdroj:	New Zealand Journal of Marine and Freshwater Research. 25:345-350
ISSN:	1175-8805 0028-8330
Popis:	Isolates of Vibrio ordalii were recovered from salmon (Oncorhynchus tshawytscha and Oncorhynchus nerka) cultured in New Zealand. The fish suffered from clinical vibriosis, from seven outbreaks in five geographically distinct areas. Affected fish showed gross signs and histopathology similar to those reported from North America. The presence of a plasmid, designated pVO1, approximately 30 kilobases (kb) in size, was seen in all V. ordalii strains examined This plasmid was identical in size and restriction endonuclease cleavage analysis to a plasmid (pMJ101) previously found in V. ordalii strains elsewhere. After togging pVO1 with the transposon Tn1 from the R plasmid RP4, attempts to isolate plasmid‐less derivatives using a variety of curing agents were unsuccessful. All of the New Zealand isolates possessed identical chromosomal DNA cleavage patterns. These patterns were only slightly different from that of the type strain but substantially different from that of the Vibrio anguillarum type strain.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1176fd89a64e4e85c006d71c86264a7f https://doi.org/10.1080/00288330.1991.9516488 Zobrazit plný text záznamu